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wave denaturing high performance liquid chromatography (dhplc  (Transgenomic)

 
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    Transgenomic wave denaturing high performance liquid chromatography (dhplc
    Wave Denaturing High Performance Liquid Chromatography (Dhplc, supplied by Transgenomic, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wave denaturing high performance liquid chromatography (dhplc/product/Transgenomic
    Average 90 stars, based on 1 article reviews
    wave denaturing high performance liquid chromatography (dhplc - by Bioz Stars, 2026-05
    90/100 stars

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    RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated

    Journal: BMC Cancer

    Article Title: High methylation levels of PCDH10 predict poor prognosis in patients with pancreatic ductal adenocarcinoma

    doi: 10.1186/s12885-019-5616-2

    Figure Lengend Snippet: RT-PCR analysis of PCDH10 in human cancer cell lines. dHPLC analysis of RT-PCR amplified cDNA shows a peak indicating the expression of PCDH10 in the breast adenocarcinoma MB-231 cells, in line with the lack of methylation of the corresponding promoter in the same cells. Conversely, no peaks are detected in Capan-2 and AsPC-1 pancreatic adenocarcinoma and in AGS gastric adenocarcinoma cells, indicating lack of PCDH10 mRNA expression in these cell lines, whose PCDH10 promoter is fully methylated

    Article Snippet: The DNA digests were separated by denaturing high-performance liquid chromatography (dHPLC) (Wave 1100, Transgenomic, Omaha, NE).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Expressing, Methylation